Importantly, do not cut across the flat surface as that will compress the tag, pinch the ear, and cause damage to the ear. A two (2) millimeter ear punch or marginal notch is recommended. While being tattooed, neonatal mice are manually restrained. To make a notch, place the punch on the edge of the ear pinna at the correct spot, in this case at the top of the right ear for the "three" in the code's unit place, and do the same - apply quick and firm pressure and then open the scissor to remove it. Next, apply pressure to close the scissor quickly and firmly to cut through the skin. Dickie, MM. If positioned too close to the head, the skin of the neck area can catch in the tag, creating irritation, pain, and restricted movement, which can then lead to self-trauma. BASIC PROTOCOL 1: Adjusting amount procedure. Tattooing is the preferred method for identifying neonatal mice. A JoVE representative will be in touch with you shortly. Now, let's move on to ear tagging, which is another inexpensive and easy-to-perform method for the individual identification of mice and rats. Ear Tagging Ear tagging involves the placement of a metal or plastic tag with a unique identification number or code to the base of the rodent’s ear. Tennessee: Cordova; 186 p. We use cookies to personalize our website and to analyze web traffic to improve the user experience. These conditions can be exacerbated by improper placement. Similarly, on the left ear, the same notches represent the tens. Please refer to our privacy policy for more information. Another limitation is that most animals must be grasped at the scruff in order to read the code, thus increasing the times they are handled. ® Ear Tagging Technique in Mice. 3. The tagging system requires the application of two identically numbered yellow plastic ear tags to calves born on a holding within 20 days of birth. Correct application of cattle … Guidelines for Tissue Collection for Genotyping Mice and Rats Purpose ... Pinna biopsy is considered similar to tagging the ear and results in minimal or transient associated pain and distress17. Ear punching can be done at age 14 days or older without anesthesia. If you want more info regarding data storage, please contact [email protected]. Avoid pulling or twisting the punch to prevent tearing the ear. Other companies may also sell them. Each of these has their advantages and disadvantages. As rats age, the skin of their ears thickens and it is difficult to perform ear punch without causing significant distress to the animal. • Place tag appropriately into applicator device. Start with the hole for 100. Animals may be less likely to struggle if the hind limbs can rest on a surface such as the cage top or a counter top. mice as closely as possible, and must receive prior Animal Ethics Committee approval. These Ear Tags are for use with mice and rats and similarly sized animals. At this time, mice are a good size to keep the tag. Please check your Internet connection and reload this page. Ear punching, commonly used for identification of rodents, involves using a special punch to either produce a small (0.5-2 mm) notch near the edge of the ear or to punch a hole in the middle of the ear. Place the right ear in the punch so that the hole of the punch is positioned away from the pinna margin. Introduction to Laboratory Animal Science and Technology. A subscription to JoVE is required to view this content.You will only be able to see the first 20 seconds. For adult rats that require identification, the use of inhalant anesthesia should be considered when performing ear punches. Ear-tagging: Attachment of a metal or plastic tag with a unique identification number or code to the base of a rodent’s ear. Ear-tagging . Methods for permanently identifying mice include ear punching, ear tagging, tattooing, and subcutaneously implanting a numerically coded microchip. Before you tattoo a mouse, disinfect its skin and the tattoo needles. Ear punching of weaning mice does not require anesthesia. Ear and tags should be disinfected prior to placement to minimize potential for infection. Ear tagging requires a special applicator to place an “earring” or metal tag with an identification number in the pinna of a mouse or rat’s ear. Ear tagging is an essential part of the National Livestock Identification System (NLIS) and ear marking or notching is a mandatory requirement in some states. Most mice will defecate within a minute of being picked up. New York, New York: Pergamon Press; 323 p. Rao GH, J Edmondson. Depending on the circumstances, either temporary or permanent methods may be used. In severe cases of infection, the animal may need to be anesthetized. There are a variety of commercially available ear tags. Unable to load video. The pointed end of the tag should be opposite the notch. A felt tip marker may be used for marking an ear or tail; such marks usually disappear in 1-2 days. Call 859-261-2035. Then, release the punch and remove it from the ear. Avoid autoclaving the ear punches, as it will eventually dull them. Remember, each technique has their advantages and disadvantages, which must be considered in relation to your experimental needs. It also has business benefits by enabling livestock to be identified on-farm, leading to improved management. The holes and notches are placed according to a predetermined code (Dickie 1975; Ingalis 1980; Stark and Ostrow 1991). In contrast, plastic tags are easy to read without having to handle the animals. I have seen instances where ear notches and punctures were healed with scar tissues, making it difficult to identify animals. Because the implants are relatively large, they are not recommended for newborns, but they are acceptable for identifying weanlings and adults. AALAS training manual series, vol 1: Assistant laboratory animal technician. This often leads to infections of the pinna, and the immediate treatment should include the removal of the tag. Please check your Internet connection and reload this page. Ear Tags Ear tags are another means for identifying mice. Stark, DM and ME Ostrow, editors. In order to remove the tag, moisten a cotton-tipped applicator with an antiseptic solution and use it to remove any debris from around the tag. Depending on your mouse identification needs and the degree of visibility you require, you can choose from several varieties of mice ear tags, including stamped, paint-filled stamped, and laser etched mouse ear tags with numbers on either … To learn more about our GDPR policies click here. We may use this info to send you notifications about your account, your institutional access, and/or other related products. UID Microchip Implant. Tags can be lost due to over-grooming, dermatitis or infection, self-trauma inflicted trying to remove tags due to intolerance, fighting, and aggressive breeding behavior. 2. Repeat the same procedure on the other ear to punch three notches - two for "20" near the outer corner and one for "50" near the inner corner, totaling the code to "70". 14 days or older No anesthesia is required when performed by trained personnel Ear tagging can lead to pressure necrosis, ulcerations, inflammation, neoplasia and infection. If the problem continues, please, An unexpected error occurred. If you have any questions, please do not hesitate to reach out to our customer success team. • Gently restrain mouse with gloved hands by scruffing the dorsum high on the neck to control both the body and the head. Ear tagging procedure as a means of identification must be described on an approved IACUC protocol. Be sure to order the applier that is appropriate for the specific size and style of ear tags being used. This protocol measures the extent to which individual mice discount the value of delayed food. The description includes training steps, which may require an average of 12-29 sessions depending on the mouse strain, and an experimental phase in which delay discounting is assessed over 5-10 weeks. Identification technique's importance increases further if the experiment is lasting over several days. And again, different combinations will cover the range of 10 through 90. Ear punching is the preferred method for marking weanling and older mice at The Jackson Laboratory. Certain experimental protocols require genotyping of neonates as early as day one, which makes it essential that these pups be permanently identified. Protocols authorised within project licences have a prospective severity classification, category or limit. Male and female BALB/c mice … Tags should be properly placed to avoid irritation or trauma. Food coloring may be used to dye a patch of fur; such marks generally last for 1-2 weeks but can be used only on albino and light-colored mice. Ear tagging. To circumvent this problem, a secondary form of identification such as a punch code on the ear opposite of the tag should be utilized. The ear tag should not be applied until the day of weaning. FDA approved tattoo pigment must be used. Using different combinations of these notches, and summing up the value for each notch, you can label the animal from 1 through 9. You've just watched JoVE's presentation demonstrating the two commonly used methods of identification in rodents: ear tags and ear punches. Before being implanted, mice are either sedated or anesthetized. Older mice are either placed in a restraining device or sedated. We use cookies to enhance your experience on our website. Tags must be placed such that they do not cause a bend in the ear, interfere with the animal's mobility, or catch on any part of the caging. ear punch, tag, microchip) are not appropriate. Postbacc, Ph.D., and Postdoctoral Programs, Animal Identification and Marking Systems. Used to analyze web traffic to improve the user experience. The same procedure is applicable to any other code or punch. The applicator tip and ear tags should be disinfected before they are used. Ear tags should be sterile or disinfected prior to placement. All rights reserved. Ergonomically, the lever punch is easier to use than thumb punch, especially for the thicker ears of the older rats. Ear Tags Tags must be appropriately size for species and age. In spite of being a quick and simple method of identification, ear punching does have some limitations. Here, we are going to discuss the two most commonly used methods: ear tagging and ear punching. The tattoo is placed on a toe or tail. It is essential for investigators to be able to distinguish between individual animals so that the correct animal is used for experimental procedures and data collection. Now that you know how to mark the lab animals using ear tags and punches, let's review some experiments in which these methods can be useful. Before using an ear punch, it should be tested to make sure it works properly and the cutting edge should be disinfected. Animal Marker is another product available which can be used on rodent’s fur. Ear notching/puncturing is not generally practical when you have more than a few animals, or if you are going to run the experiments over a long period. The codes are easily distinguished from one another by a computer. You should now understand the ear punch code, know the different types of commercially available rodent ear tags, and should be able to accurately perform these procedures without causing significant animal discomfort. Your access has now expired. • Soak ear tags in alcohol or other disinfectant prior to application. Keeping Records. Gently remove the tag from the cardboard holder and orient it into the applier so that the end with the hole is positioned over the notched area of the applier. Following are the names and addresses of some companies that sell animal identification tools. Then, using wire cutters, cut across the top of the tag loop from edge to edge. Following application, we will also discuss removal of the ear tag, which is usually performed in case of an infection due to the tag application. Microchips are about the size of a grain of rice and transmit a unique alphanumeric code for each mouse. Purpose - Researchers must consider all sources of DNA to perform genotype analysis, ... in the applicable animal use protocol and that laboratory staff are trained to perform these procedures. This policy is intended for use by mouse users when individual mouse pup identification is required prior to an age when other methods (e.g.

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